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An analytical technique used to separate, identify, and quantify components in a mixture. In peptide analysis, HPLC is the gold standard for purity assessment. The sample is dissolved in a solvent and pumped through a column packed with adsorbent material under high pressure. Different components separate based on their interactions with the column material, producing a chromatogram where each peak represents a distinct component. Purity is calculated as the percentage of the target peptide peak area relative to all detected peaks.